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Form of Proxy

TAYSHA GENE THERAPIES, INC.

Notes

No. Proposition For Against Abstain
1

DIRECTOR Sean P. Nolan

2

DIRECTOR L. Sepp-Lorenzino Ph.D

3

To ratify the selection by the Audit Committee of the Board of Directors of Deloitte & Touche LLP as the independent registered public accounting firm of the Company for the year ending December 31, 2026.

4

To approve, on a non-binding advisory basis, the compensation of the Companys named executive officers.

5

To indicate, on a non-binding advisory basis, the preferred frequency of future stockholder advisory votes on the compensation of the Companys named executive officers.