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Form of Proxy

Q32 BIO INC.

Notes

No. Proposition For Against Abstain
1

Election of Class I Director to serve until the 2028 Annual Meeting of stockholders and until his successor has been duly elected and qualified, or until his earlier death, resignation, or removal: David Grayzel, M.D.

2

Election of Class I Director to serve until the 2028 Annual Meeting of stockholders and until his successor has been duly elected and qualified, or until his earlier death, resignation, or removal: Isaac Manke, Ph.D.

3

Election of Class I Director to serve until the 2028 Annual Meeting of stockholders and until his successor has been duly elected and qualified, or until his earlier death, resignation, or removal: Diyong Xu

4

To approve an amendment to our Restated Certificate of Incorporation, as amended, to limit the liability of certain officers as permitted by amendments to Delaware law.

5

To ratify the appointment of Ernst & Young LLP as our independent registered public accounting firm for the fiscal year ending December 31, 2025.

6

To approve, on a non-binding, advisory basis, the compensation of our named executive officers.

7

To approve, on a non-binding, advisory basis, the preferred frequency of future advisory votes on compensation of our named executive officers.

8

To approve the adjournment of the Annual Meeting to the extent there are insufficient votes at the Annual Meeting to approve Proposal No. 2.