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Form of Proxy

STOKE THERAPEUTICS, INC.

Notes

No. Proposition For Against Abstain
1

DIRECTOR Edward M. Kaye, M.D.

2

DIRECTOR Seth L. Harrison, M.D.

3

DIRECTOR A. O. Tzianabos, Ph.D.

4

To ratify the appointment of KPMG LLP as our independent registered public accounting firm for the fiscal year ending December 31, 2025.

5

To approve, on an advisory basis, the compensation of the Companys named executive officers.

6

To indicate, on an advisory basis, the preferred frequency of future stockholder advisory votes to approve the compensation of the Companys named executive officers.